In 2002, ASTM International issued a standard specification for BioDiesel fuel called D6751. This specification states that the only form of BioDiesel that can be legally resold for commercial operations must meet ASTM specifications.

ASTM Test Method D6584 is the Gas Chromatography analysis of Biodiesel for Free and Total Glycerines. It requires that a two-internal standard, five-point calibration curve be generated for free Glycerine, Monoglycerides, Diglycerides and Triglycerides achieving a linearity of 0.99 r2. The analytical standards must be derivatized from the free acid form to a methyl ester form so that they will move through a gas chromatographic column prior to analysis.